Applications
Column
Label-free and 3D cell culture
Label-free assay
Laboratory research and applications have always benefitted by the utility of fluorescence based probes to evaluate and markers or processes for better understanding of processes. As beneficial these have been in life sciences, these also pose limitations on assessing changes at cellular/molecular levels , because, fluorescence staining is not only labor-intensive and costly, from fixation to observation, but also makes it difficult to observe the dynamic changes occurring in cells over the course of culture. In addition, exogenous expression of fluorescent protein may affect the ground state of cells. In recent years, immense advances have been made in the area of regenerative medicine and cancer research. These advances have been possible by assessing the changes at cellular or molecular level by studying dynamic changes in cells. To this end, there is an ever-increasing need to observe and manage cellular changes without having the need to exogenously introduce markers tagged with fluorescent probes of interest or introduce fluorescent probes into the cells. Label-free assays have, therefore, rapidly grown to be of high interest in research and applications. SCREEN imaging technology, Cell3iMager series have proven to be ideal systems in research and development for performing label-free assays.
Possibility of 3D cell culture
There has been rapid advance in in vitro and ex vivo based laboratory research with 3D cell culture gaining relevance in cell based assays. It has already been shown that 3D cell cultures reflect in vivo environment, therefore, highly important in assessing the phenotypic changes. It is also well known that 3D cell cultures pose a complicated three-dimensional structure, in order to analyze biological phenomena using the in vitro system, it is important to utilize technology to evaluate the complex 3D structures as well as tissues. Over the past years, 3D cell culture platforms are being used in disease modelling drug discovery, early development. Key areas such as , regenerative medicine and cancer research are utilizing three-dimensional cell culture assays to better understand the biological processes.
Contributions to Research with our Cell3iMager Series
The Cell3iMager series specializes in label-free 3D imaging and quantitative analysis of 3D cultured organoids and spheroids. In particular, the versatile and robust Cell3imajer technology exhibits key aspects in useful in high throughput drug discovery and early development.
3D-cultured cells, a fundamental technology that supports regenerative medicine and drug discovery research
3D Culture Cells
(Single spheroid)
Imaging with Cell3iMager duos
3D Culture Cells
(Multi spheroid)
Imaging with Cell3iMager duos
3D Culture Cells
(Single spheroid)
Imaging with Cell3iMager Estier
Application note
Collaboration
"Development of spheroid-based anticancer drug screening method using high-speed cell culture scanner" (CC-5000)
(Collaborative research with Nagahama Institute of Bio-Science and Technology Mizukami Laboratory)
"Rapid production and size assessment of Embryoid Bodies ( EBs )" (CC-5000)
(Collaborative research with InShero AG)
"High-Speed 3D Cell Scanner —Perfect for the Study of Complex 3D MCTS Models" (CC-5000)
(Collaborative research with MD Anderson Cancer Center)
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Cell Viability and Cytotoxicity Assay
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Single cell cloning hybridoma
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Single cell cloning
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3D Cell Culture by using FCeM
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Assessing the growth of 3D spheroids cultured in Matrigel
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Analysis of microscope images
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Counting fluorescence stained adherent cells
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Evaluation of hypoxia level in 2D/3D-cultured cells
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Cell Proliferation
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Label free colony-forming units assay
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Confluency
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Neuronal differentiation
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Suspension cultured cell
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Tissue sections
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Label free analysis of spheroids in microwells
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Deep Learning for cell classification
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Analysis of spheroids in microwells
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Organelle Staining
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Aniti-Cancer Drug Screening
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LiveDead Assay
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Invasion Assay
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Label free analysis of spheroids in microwells